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irf4 primary ab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc irf4 primary ab
    Figure 7. Regulation of <t>IRF4</t> in CD8+ T cells by NAC1. CD8+ T cells were isolated from WT or NAC1−/−mice and analyzed for expression of IRF4. (A) Protein expression by Western blot. For the day 0 sample, T cells were not activated. For the day 3 sample, T cells were activated and cultured for 3 days. (B) mRNA expression by Q-PCR. CD8+ T cells had been cultured for 3 days, and then RNA was extracted, and qPCR was performed (ns, p > 0.05). (C) CHIP-seq analysis. WT CD8+ T cells were isolated from mice and CHIP was performed with anti-NAC1 and anti-IgG Abs (Input control). The sequenced data was visualized by IGV.
    Irf4 Primary Ab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/irf4+primary+ab/IRF-4+Antibody/pm36016335-50-1-10
    Average 95 stars, based on 155 article reviews
    irf4 primary ab - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Expression of NAC1 Restrains the Memory Formation of CD8 + T Cells during Viral Infection."

    Article Title: Expression of NAC1 Restrains the Memory Formation of CD8 + T Cells during Viral Infection.

    Journal: Viruses

    doi: 10.3390/v14081713

    Figure 7. Regulation of IRF4 in CD8+ T cells by NAC1. CD8+ T cells were isolated from WT or NAC1−/−mice and analyzed for expression of IRF4. (A) Protein expression by Western blot. For the day 0 sample, T cells were not activated. For the day 3 sample, T cells were activated and cultured for 3 days. (B) mRNA expression by Q-PCR. CD8+ T cells had been cultured for 3 days, and then RNA was extracted, and qPCR was performed (ns, p > 0.05). (C) CHIP-seq analysis. WT CD8+ T cells were isolated from mice and CHIP was performed with anti-NAC1 and anti-IgG Abs (Input control). The sequenced data was visualized by IGV.
    Figure Legend Snippet: Figure 7. Regulation of IRF4 in CD8+ T cells by NAC1. CD8+ T cells were isolated from WT or NAC1−/−mice and analyzed for expression of IRF4. (A) Protein expression by Western blot. For the day 0 sample, T cells were not activated. For the day 3 sample, T cells were activated and cultured for 3 days. (B) mRNA expression by Q-PCR. CD8+ T cells had been cultured for 3 days, and then RNA was extracted, and qPCR was performed (ns, p > 0.05). (C) CHIP-seq analysis. WT CD8+ T cells were isolated from mice and CHIP was performed with anti-NAC1 and anti-IgG Abs (Input control). The sequenced data was visualized by IGV.

    Techniques Used: Isolation, Expressing, Western Blot, Cell Culture, ChIP-sequencing, Control

    Related Articles

    Isolation:

    Article Title: Expression of NAC1 Restrains the Memory Formation of CD8 + T Cells during Viral Infection.
    Article Snippet: The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).. The IRF4 primary Ab used is rabbit anti-mouse IRF4 Ab (CST #62834T).. The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).

    Article Title: Expression of NAC1 Restrains the Memory Formation of CD8 + T Cells during Viral Infection
    Article Snippet: The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).. The IRF4 primary Ab used is rabbit anti-mouse IRF4 Ab (CST #62834T).. The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).

    Expressing:

    Article Title: Expression of NAC1 Restrains the Memory Formation of CD8 + T Cells during Viral Infection.
    Article Snippet: The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).. The IRF4 primary Ab used is rabbit anti-mouse IRF4 Ab (CST #62834T).. The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).

    Article Title: Expression of NAC1 Restrains the Memory Formation of CD8 + T Cells during Viral Infection
    Article Snippet: The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).. The IRF4 primary Ab used is rabbit anti-mouse IRF4 Ab (CST #62834T).. The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).

    Western Blot:

    Article Title: Expression of NAC1 Restrains the Memory Formation of CD8 + T Cells during Viral Infection.
    Article Snippet: The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).. The IRF4 primary Ab used is rabbit anti-mouse IRF4 Ab (CST #62834T).. The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).

    Article Title: Expression of NAC1 Restrains the Memory Formation of CD8 + T Cells during Viral Infection
    Article Snippet: The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).. The IRF4 primary Ab used is rabbit anti-mouse IRF4 Ab (CST #62834T).. The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).

    Cell Culture:

    Article Title: Expression of NAC1 Restrains the Memory Formation of CD8 + T Cells during Viral Infection.
    Article Snippet: The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).. The IRF4 primary Ab used is rabbit anti-mouse IRF4 Ab (CST #62834T).. The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).

    Article Title: Expression of NAC1 Restrains the Memory Formation of CD8 + T Cells during Viral Infection
    Article Snippet: The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).. The IRF4 primary Ab used is rabbit anti-mouse IRF4 Ab (CST #62834T).. The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).

    ChIP-sequencing:

    Article Title: Expression of NAC1 Restrains the Memory Formation of CD8 + T Cells during Viral Infection.
    Article Snippet: The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).. The IRF4 primary Ab used is rabbit anti-mouse IRF4 Ab (CST #62834T).. The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).

    Article Title: Expression of NAC1 Restrains the Memory Formation of CD8 + T Cells during Viral Infection
    Article Snippet: The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).. The IRF4 primary Ab used is rabbit anti-mouse IRF4 Ab (CST #62834T).. The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).

    Control:

    Article Title: Expression of NAC1 Restrains the Memory Formation of CD8 + T Cells during Viral Infection.
    Article Snippet: The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).. The IRF4 primary Ab used is rabbit anti-mouse IRF4 Ab (CST #62834T).. The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).

    Article Title: Expression of NAC1 Restrains the Memory Formation of CD8 + T Cells during Viral Infection
    Article Snippet: The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).The protein samples were then collected and quantified using BCA protein assay (Thermo Fisher #23225).. The IRF4 primary Ab used is rabbit anti-mouse IRF4 Ab (CST #62834T).. The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).The β-ACTIN primary Ab utilized is rat anti-mouse ACTIN Ab (BioLegend #664802).



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    irf4 primary ab  (Cell Signaling Technology Inc)
    95
    Cell Signaling Technology Inc irf4 primary ab
    Figure 7. Regulation of <t>IRF4</t> in CD8+ T cells by NAC1. CD8+ T cells were isolated from WT or NAC1−/−mice and analyzed for expression of IRF4. (A) Protein expression by Western blot. For the day 0 sample, T cells were not activated. For the day 3 sample, T cells were activated and cultured for 3 days. (B) mRNA expression by Q-PCR. CD8+ T cells had been cultured for 3 days, and then RNA was extracted, and qPCR was performed (ns, p > 0.05). (C) CHIP-seq analysis. WT CD8+ T cells were isolated from mice and CHIP was performed with anti-NAC1 and anti-IgG Abs (Input control). The sequenced data was visualized by IGV.
    Irf4 Primary Ab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/irf4+primary+ab/IRF-4+Antibody/pm36016335-50-1-10
    Average 95 stars, based on 1 article reviews
    irf4 primary ab - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 7. Regulation of IRF4 in CD8+ T cells by NAC1. CD8+ T cells were isolated from WT or NAC1−/−mice and analyzed for expression of IRF4. (A) Protein expression by Western blot. For the day 0 sample, T cells were not activated. For the day 3 sample, T cells were activated and cultured for 3 days. (B) mRNA expression by Q-PCR. CD8+ T cells had been cultured for 3 days, and then RNA was extracted, and qPCR was performed (ns, p > 0.05). (C) CHIP-seq analysis. WT CD8+ T cells were isolated from mice and CHIP was performed with anti-NAC1 and anti-IgG Abs (Input control). The sequenced data was visualized by IGV.

    Journal: Viruses

    Article Title: Expression of NAC1 Restrains the Memory Formation of CD8 + T Cells during Viral Infection.

    doi: 10.3390/v14081713

    Figure Lengend Snippet: Figure 7. Regulation of IRF4 in CD8+ T cells by NAC1. CD8+ T cells were isolated from WT or NAC1−/−mice and analyzed for expression of IRF4. (A) Protein expression by Western blot. For the day 0 sample, T cells were not activated. For the day 3 sample, T cells were activated and cultured for 3 days. (B) mRNA expression by Q-PCR. CD8+ T cells had been cultured for 3 days, and then RNA was extracted, and qPCR was performed (ns, p > 0.05). (C) CHIP-seq analysis. WT CD8+ T cells were isolated from mice and CHIP was performed with anti-NAC1 and anti-IgG Abs (Input control). The sequenced data was visualized by IGV.

    Article Snippet: The IRF4 primary Ab used is rabbit anti-mouse IRF4 Ab (CST #62834T).

    Techniques: Isolation, Expressing, Western Blot, Cell Culture, ChIP-sequencing, Control